Top of pageResultsCharacterization of deprivation (neglect)induced deathIf growth medium is replaced by buffered saline, HeLa cells in culture undergo apoptosis within 6 (neglectinduced death). Apoptotic nuclear damage was determined using Hoechst 33 to reveal changes in morphology and the TUNEL assay to detect doublestranded breaks in cells (Figure 1a). The TUNEL assay gave comparable results to that obtained with Hoechst 33 (Figure 1b), and in all subsequent analysis, the assessment of nuclear morphology using Hoechst has been presented as the readout of damage.Half the time, people who live in hotels are either eccentric millionaires or adorable, adventureprone children. The rest of the time, they are usually creepy drifters. Sometimes it hard to tell which is which, so corpsewary travelers should play it safe and avoid hotels that court the longterm trade.

Right now, one in three African American students are dropping out of high school. Only one in five African Americ